Reactive oxygen species (ROS) are chemically reactive molecules which can wreak havoc in biological systems. They can be generated through exposure to adverse environmental stimuli, such as UV light, exposure to environmental pollutants, and even taken in with a normal diet. Furthermore, some of the bodies normal functions can generate potentially harmful ROS, including mitochondrial metabolism and other everyday cellular redox reactions.
Once formed ROS can attach vulnerable parts of the cells, including inactivating proteins and lipids. But perhaps the most serious target of ROS are nucleic acids. ROS modifications to DNA can result in DNA mutations which can either be fatal to the cell or result in the transformation of a normal cell to a cancerous cell. In addition to cancer, ROS exposure is also associated with accelerating the aging process, inflammatory disorders, and certain degenerative diseases.
In light of the extreme damage that ROS can do to biological systems, the value of antioxidants which can combat and neutralize ROS can not be understated. Our laboratory services include assays which can screen materials to either determine if they have antioxidant activity and how well these materials function to prevent intracellular ROS formation and ROS mediated cellular damage.
Once formed ROS can attach vulnerable parts of the cells, including inactivating proteins and lipids. But perhaps the most serious target of ROS are nucleic acids. ROS modifications to DNA can result in DNA mutations which can either be fatal to the cell or result in the transformation of a normal cell to a cancerous cell. In addition to cancer, ROS exposure is also associated with accelerating the aging process, inflammatory disorders, and certain degenerative diseases.
In light of the extreme damage that ROS can do to biological systems, the value of antioxidants which can combat and neutralize ROS can not be understated. Our laboratory services include assays which can screen materials to either determine if they have antioxidant activity and how well these materials function to prevent intracellular ROS formation and ROS mediated cellular damage.
Antioxidant Activity Assays
- DPPH Assay
- FRAP Assay
- ORAC Assay
Detection of Specific ROS
- Singlet Oxygen
- Hydroxyl Radicals
- Superoxide Radicals
- Peroxide Radicals
- Lipid Peroxides
- Peroxyl Radicals
Detection of Intracellular ROS Formation/Damage
- 2',7'-Dichlorofluorescein Reactive Oxygen Species Detection Dye
- Lipid Peroxidation Assays
- DNA 8-oxoguanine formation
- Intracellular Peroxy Nitrate Formation
Please note that only the typical targets are listed. If there are other antioxidant tests or markers that your company has an interest in please let us know and we can accommodate you. Please contact us for specific pricing and study timing.