Irritation: Epiderm and EpiOcular Assays
Standard irritation assays make use of human three dimensional tissue equivalents (MatTek's EpiDerm and EpiOcular tissues). The models allow for test materials to be applied topically, which facilitates their use with solid and insoluble materials. The endpoint for the assay is the determination of an EC50 time (the time that it takes for tissue viability to be reduced to 50%), from which the irritation potential of the material can be predicted. Additional endpoints include assays for chemical mediators of inflammation such as IL-1a, PGE2, IL-6 or IL-8.
Phototoxicity
A material is considered to be phototoxic if when applied to the body it exerts a toxic effect or an enhanced toxic effect (the material becomes toxic at a concentration below its normal toxic levels) after the material has been exposed to light. Phototoxic effects of materials can be observed when the material is applied topically to the skin, or if the material is taken systemically and becomes exposed to light via irradiation through the skin.
Phototoxicity testing is traditionally conducted using the mouse 3T3 fibroblast cell line, although the test can also be conducted using human cultured cells or using MatTek EpiDerm tissues. Phototoxicity testing involves comparing changes in the viability of cells or tissues after treatment with the test material both in the presence and absence of light.
Phototoxicity testing is traditionally conducted using the mouse 3T3 fibroblast cell line, although the test can also be conducted using human cultured cells or using MatTek EpiDerm tissues. Phototoxicity testing involves comparing changes in the viability of cells or tissues after treatment with the test material both in the presence and absence of light.
Please contact us for specific pricing and study timing.